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Dharmacon™ Edit-R™ has been featured in over 20 peer-reviewed publications since 2015

Check out our recommended reading list! It includes the Dharmacon Edit-R CRISPR-Cas9 system, and custom crRNAs and RNAs, used for gene editing in mammalian cells and in other exciting, creative applications.

Did you know that synthetic crRNA:tracrRNA with Cas9 protein can be delivered as a ribonucleoprotein for functional genetic screening in primary human T cells (Hultquist, 2016)?

Or, for instance, that CRISPR-Cas9 with synthetic RNAs can be used for identification of plasmids that confer bacterial resistance (Müller, 2016)?

Edit-R has also been successfully applied to uncover gene functions in nematodes (Delaney, 2017 and Paix, 2015).

Publications with Dharmacon Edit-R CRISPR-Cas9 reagents

2017
  1. C. E. Delaney, A. T. Chen, et al. A histone h6 lysine 20 methyltransferase couples environmental cues to sensory neuron control of developmental plasticity. Development. 144, 1273-1282 (2017).
  2. N. Maio, K. S. Kim, et al. A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III. Cell Metab. 25, 945-953, e6 (2017).
  3. L. J. Rupp, K. Schumann, et al. CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells. Sci Rep. 7, 737 (2017).
  4. Ž. Strezoska, M. Perkett, et al. High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries. J. Biotechnol. 251, 189-200 (2017).
  5. X. M. van Wijk, S. Döhrmann, et al. Whole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin α2 as a Host Factor for Bacterial Invasion. MBio. 8, e02128-16 (2017).
2016
  1. T. A. Aguilera, M. Rafat, et al.. Reprogramming the immunological microenvironment through radiation and targeting Axl. Nat Commun. 7, 13898 (2016).
  2. K. He, E. Chou, et al. Conjugation and evaluation of triazole-linked single guide RNA for CRISPR-Cas9 gene editing. ChemBioChem. 17, 1809–1812 (2016).
  3. R. Eggenschwiler, M. Moslem, et al. Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase. Sci Rep. 6, 38198 (2016).
  4. J. F. Hultquist, K. Schumann, et al. A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells. Cell Reports 17, 1438–1452 (2016).
  5. M. L. Kelley, Ž. Strezoska, et al. Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing. J. Biotechnol. 233, 74–83 (2016).
  6. J. McCaffrey, J. Sibert, et al. CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis. Nucleic Acids Res. 44, e11 (2016).
  7. V. Müller, F. Rajer, et al. Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping. Sci Rep. 6, 37938 (2016).
  8. A. Paix, H. Schmidt, et al. Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs. Nucleic Acids Res. 44, e128 (2016).
  9. J. Tan, S. E. Martin. Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening. PLOS ONE 11, e0168968 (2016).
2015
  1. E. M. Anderson, A. Haupt, et al. Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity. J. Biotechnol. 211, 56-65 (2015).
  2. R. Barrangou, A. Birmingham, et al. Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference. Nucleic Acids Res. 43, 3407–3419 (2015).
  3. H. Ogiwara, M. Sasaki, et al. Targeting p300 addiction in CBP-deficient cancers causes synthetic lethality by apoptotic cell death due to abrogation of MYC expression. Cancer Discov. 6, 430-445 (2015).
  4. S. Opp, D. A. S. A. Vieira, et al. MxB Is Not Responsible for the Blocking of HIV-1 Infection Observed in Alpha Interferon-Treated Cells. J. Virol. 90, 3056-3064 (2015).
  5. A. Paix, A. Folkmann, et al. High efficiency, homology-directed genome editing in Caenorhabditis elegans using CRISPR-Cas9 Ribonucleoprotein complexes. Genetics 201, 47-54 (2015)
  6. G. Sivan, P. Ormanoglu, et al. Identification of Restriction Factors by Human Genome-Wide RNA Interference Screening of Viral Host Range Mutants Exemplified by Discovery of SAMD9 and WDR6 as Inhibitors of the Vaccinia Virus K1L-C7L- Mutant. MBio. 6, e01122 (2015).
  7. W. Deng, X. Shi, et al. CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc Natl Acad Sci U S A. 112, 11870–11875 (2015).

Find out about all of these applications on our CRISPR-Cas9 Recommended Reading List »

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