Design and order libraries of synthetic reagents
- Our online tool offers the flexibility to configure a library for your unique experimental needs - no quote required!
- Choose synthetic reagents from our algorithm-optimized, predesigned products lines, including Dharmacon™ siRNA and microRNA, or Edit-R™ CRISPR sgRNA and crRNA
- For vector-based reagents, please see instructions for designing Cherry-pick clone libraries
- Add standard controls anywhere on the plate
- 100% customizable plate layout
When Horizon pre-defined RNAi Screening Libraries or Edit-R crRNA Libraries don't match your experimental requirements, you can make your own custom library with ease, speed and economy.
You are able to cherry-pick from our premier siRNA and microRNA product lines and Edit-R predesigned crRNA reagents for human and mouse reagents to create the ideal collection for your particular needs.
- Load gene identifiers for your desired products
- NCBI Gene ID or Gene Symbol
- miRBase mature name or Accession
- Horizon product catalog numbers
Use the Library Plater to order these products in 96- or 384-well plates
|Product lines||siRNA: ON-TARGETplus, siGENOME, Accell, or Lincode microRNA: miRIDIAN Mimics or Inhibitors crRNA: Edit-R crRNA (may not be combined with RNAi reagents in the same library)|
|Product formats||siRNA: SMARTpool or up to 4 individual siRNAs microRNA: Hairpin Inhibitor or Mimic Learn more about RNAi Product Formats crRNA: Up to 5 individual crRNA per gene|
|Species||Human or Mouse|
|Plate type||96 well siRNA/microRNA or crRNA plates; 20 well minimum 384-well siRNA/microRNA or crRNA plates; 40 well minimum|
|Quantity per well||0.1, 0.25, 0.5, 1 or 2 nmol (1 or 2 nmol per well only available for 96-well plates)|
These options are available but cannot be ordered online. They require a custom quote
|Reverse Transfection Format (RTF)||Ready-to-use siRNA/microRNA screening plates (min. 8 replicates) dispensed at 6.25 pmol per well in 96-well culture plate. Clear, black-or white-walled plates available. Not available for crRNA|
|Labcyte Echo-qualified 384-well plates||Part# PP-0200. LDV plates not available.|
Which siRNA is right for you?
Horizon offers four complete pre-designed product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.
|Cost-effective, efficient silencing||High specificity for reduced off-targets + efficient silencing||Highly specific knockdown of long noncoding RNA (lncRNA)||Target silencing in difficult-to-transfect cells|
|siGENOME siRNA||ON-TARGETplus siRNA||Lincode siRNA||Accell siRNA|
|Pre-designed for Human, Mouse and Rat protein-coding genes|
|Pre-designed for Human and Mouse long noncoding RNA (lncRNA)|
|Recommended for transfectable mammalian cells in culture|
|Recommended for neuronal, suspension, primary and other difficult-to-transfect cells|
|Recommended transfection reagent||DharmaFECT transfection reagent||DharmaFECT transfection reagent||DharmaFECT transfection reagent||None required|
|Available as SMARTpool reagent|
|Available as four individual siRNAs|
|Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs|
|Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake||Selective application when thermodynamic analysis indicates it necessary for favorable antisense RISC loading|
|Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity|
|Modifications to facilitate cellular uptake without separate transfection reagents|
|Stabilizing modifications to prevent nuclease-mediated degradation|
|Sequence information provided with purchase|
SMARTpool siRNA reagent
A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus SMARTpool are guaranteed† to silence by 75% or better.
Select 1, 2, 3 or 4 individual siRNAs† per gene.
microRNA Mimics and Hairpin Inhibitors
Available for human and mouse microRNAs in miRBase v.21
Select up to 5 individual crRNAs per gene. May not be combined with RNAi products on the same plate.
†Our siRNA knockdown guarantee:
siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100nM siRNA).
Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25nM working concentration.
Human siRNA Libraries
Choose from three siRNA product lines, including two whole-genome collections, to find a SMARTpool or individual siRNA library suitable for your discovery efforts.
Mouse siRNA Libraries
Select ON-TARGETplus for unsurpassed specificity. Select siGENOME libraries for the most up-to-date mouse siRNAs available for optimal gene target coverage.
Lincode siRNA Libraries
Choose a collection of Human or Mouse siRNAs based on RefSeq lncRNA genes with NR_ Accession numbers
miRIDIAN microRNA Libraries
Mimic and Hairpin Inhibitor libraries are available for human, mouse, and rat microRNAs. Provided in 96-well plates for high-throughput phenotypic screening.
RTF siRNA Libraries
Human and mouse SMARTpool siRNA libraries in a novel pre-dispensed format to enable RNAi screening without the need for high-throughput automation.
Edit-R crRNA Libraries
Plated pre-defined collections of four crRNA per gene targeting popular human and mouse gene families for arrayed knockout screening