Download our new eBook to get up to speed on recent advancements in custom oligonucleotide synthesis.
Revvity molecular reference standards are available for both germline and somatic variants. In this article we cover why this distinction is important, and what it means for the allele frequencies of variants in these two types of controls.
With the requirement of thorough in vitro assays and high throughput screening technologies to ensure drug safety and efficacy, make sure you're fully ready for when you submit your IND application by assessing immunogenicity with an MLR assay
When making a knockout cell line using CRISPR, western blotting is a useful tool to help in the verification of the cell line. However, correct interpretation of results is crucial. Here we summarize the possible fates of a protein after knockout and explore how best to validate a knockout cell line
Learn about how T cell exhaustion poses a significant challenge in immuno-oncology and the way epigenetic mechanisms can help develop strategies for reinvigorating exhausted T cells.
A study where we have employed orthogonal target validation strategies with 3 orthogonal LOF technologies (CRISPRko, CRISPRi, and siRNA) with genes involved in maintenance of the mesenchymal-to-epithelial transition (MET).
Given the enthusiasm for adopting CRISPR-based approaches, let’s take a look at the known limiting factors in using arrayed lentiviral resources, and what we can learn from this work as CRISPR arrayed screening advances as a discovery method.
Learn about considerations around the development of the Dharmacon™ Edit-R™ CRISPRko algorithm for functional and specific guide RNA
Reliable and consistent quality reagents are critical to experimental success and reproducibility. Learn about how Dharmacon RNA and DNA oligonucleotide synthesis delivers that level of quality, and explore troubleshooting tips for investigating unexpected results in your experiments.
The reasoning and research behind our Dharmacon™ CRISPRi platform recently published in The CRISPR Journal.