CRISPR-Cas9 gene editing experiments require the use of negative control samples in order to accurately distinguish biological effects of sgRNA-induced editing of targeted genomic DNA from non-specific effects. All Edit-R Non-targeting Controls are designed to have a minimum of three mismatches or gaps to all potential PAM-adjacent targets in human or mouse genomes.

Ten different negative control CRISPR RNA sequences are designed and provided as glycerol stocks and as 50 µL* (2 x 25 µL) purified, concentrated lentiviral particles. Due to the biological variability inherent in different cells, time points, assays and conditions, we recommend testing at least two non-targeting sgRNAs to identify the best negative control for establishing an effective baseline for your particular experiment. Edit-R Lentiviral sgRNA Non-targeting Controls are:

• CRISPR guide RNA sequences cloned into the same optimized lentiviral expression backbone as Edit-R gene targeting sgRNAs;
• available as glycerol stocks and as purified, concentrated lentiviral particles which avoids toxicity due to cellular debris and nucleases resulting from the packaging reaction and present in supernatants;
• proprietary alignment tools are used to verify at least three mismatches or gaps to any potential target in human or mouse genomes; and
• verified bioinformatically to not be homologous to any genes in the human or mouse genomes.

*Bulk volumes of lentiviral sgRNA control particles are available upon request