Edit-R lentiviral sgRNA non-targeting controls
Lentiviral sgRNA constructs bioinformatically designed and validated to not target any gene in human or mouse genomes.
Negative control sgRNAs to establish experimental baselines and to distinguish sequence-specific biological effects from non-specific effects. Available as high-titer purified lentiviral particles and glycerol stock formats (mouse designs only).
Please note: The glycerol stock for human designs was discontinued January 19, 2024. Should you have any questions please contact your local account representative. Additionally, lentiviral products for human designs have been updated with a new and improved vector backbone.
CRISPR-Cas9 gene editing experiments require the use of negative control samples in order to accurately distinguish biological effects of sgRNA-induced editing of targeted genomic DNA from non-specific effects. All Edit-R Non-targeting Controls are designed to have a minimum of three mismatches or gaps to all potential PAM-adjacent targets in human or mouse genomes.
Ten different negative control CRISPR RNA sequences are designed and provided as glycerol stocks (mouse designs only) and as 50 µL* (2 x 25 µL) purified, concentrated lentiviral particles. Due to the biological variability inherent in different cells, time points, assays and conditions, we recommend testing at least two non-targeting sgRNAs to identify the best negative control for establishing an effective baseline for your particular experiment. Edit-R Lentiviral sgRNA Non-targeting Controls are:
- CRISPR guide RNA sequences cloned into the same optimized lentiviral expression backbone as Edit-R gene targeting sgRNAs;
- available as glycerol stocks (mouse designs only) and purified, concentrated lentiviral particles which avoids toxicity due to cellular debris and nucleases resulting from the packaging reaction and present in supernatants;
- proprietary alignment tools are used to verify at least three mismatches or gaps to any potential target in human or mouse genomes; and
- verified bioinformatically to not be homologous to any genes in the human or mouse genomes.
*Bulk volumes of lentiviral sgRNA control particles are available upon request
