Edit-R lentiviral sgRNA negative controls
Lentiviral sgRNA constructs bioinformatically designed and validated to not target any gene in human or mouse genomes.
Negative control sgRNAs to establish experimental baselines and to distinguish sequence-specific biological effects from non-specific effects. Available as high-titer purified lentiviral particles and glycerol stock formats (mouse designs only).
Please note: The glycerol stock for human designs was discontinued January 19, 2024. Should you have any questions please contact your local account representative. Additionally, lentiviral products for human designs have been updated with a new and improved vector backbone.
AAVS1/Rosa26 cutting controls are designed to target regions of the genome that do not result in functional knockout of a protein or known phenotypic changes. These regions are sometimes known as safe harbor regions. These guides are validated for mismatch detection assays and are recommended for screening applications as an additional control that results in Cas9 cutting without a phenotypic readout.
Non-targeting controls are recommended as negative controls for experiments using synthetic sgRNA. All Edit-R non-targeting controls are designed to have a minimum of three mismatches or gaps to all potential PAM-adjacent targets in the human or mouse genomes. Changes in viability or gene expression levels in cells treated with these controls likely reflect a baseline cellular response that can be compared to the levels in cells treated with target-specific sgRNAs.
