Colorectal Cancer Panel ctDNA reference standard
Colorectal cancers (CRC) are the second most leading cause of cancer deaths worldwide (2020). To aid in the early diagnosis and direct treatment trajectories for CRC patients, molecular/genetic profiling is invaluable. Our new CRC circulating tumor DNA (ctDNA) reference panel is made with a unique blend of engineered cell lines with clinically relevant mutations specific to CRCs. This panel mimics ctDNA and can be used as a reference material to validate NGS and other molecular biology workflows for the detection of key actionable mutations in genes like AKT1, APC, BRAF, KRAS, PIK3CA and TP53 (please refer table below to see full list of genes). The allelic frequencies for the variants in the panel are as low as 1.25% with a scope of diluting further using our matched Negative Control - HD843, enabling assessment of minimal residual disease (MRD) typically replicating post-surgery and post-adjuvant treatment conditions. This reference material can also be used to validate companion diagnostic (CDx) tests which are used for tailoring treatments depending on the genetic make-up of the tumors for improved prognosis. The CRC ctDNA reference panel is a blend of highly characterized, cell line derived, clinically relevant mutations manufactured under ISO 9001:2015 and ISO 13485:2016.
Technical data
Format: ctDNA
Genes covered: ABL1, ALK, AKT1, APC, BRAF, PIK3CA, KRAS, NRAS, PTEN, TP53
Allelic frequencies: 1.25% - 8.00%
Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Product information
Fragment size: 160 bp
Unit size: 350 ng per vial
Concentration: 20 ng/µl
Verified mutations:
| Gene | DNA sequence change | Amino acid change | Expected allelic frequency (%) |
|---|---|---|---|
| ABL1 | c.1001C>T | p.T315I | 1.25 |
| ALK | c.3522C>A | p.F1174L | 2.50 |
| AKT1 | c.49G>A | p.E17K | 1.25 |
| APC | c.8140C>T | p.R2714C | 8.00 |
| BRAF | c.1799T>A | p.V600E | 1.25 |
| PIK3CA | c.1624G>A | p.E542K | 1.88 |
| KRAS | c.35G>A | p.G12D | 2.50 |
| NRAS | c.181C>A | p.Q61K | 1.25 |
| PTEN | c.388C>T | p.R130* | 2.50 |
| TP53 | c.722C>T | p.S241F | 2.50 |
General information
Storage: 4°C
Expiration: See all product shelf life information
Intended use: For assay developers, molecular diagnostic labs (Research use only)
Quality control
Fragmentation size: D1000 DNA ScreenTape assay
Allelic frequency: Droplet Digital™ PCR
Quantification: Qubit® dsDNA BR assay (Invitrogen)
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Additional Resources
Certificate of analysis
Safety data sheets
Selection guides
Reference Standards
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At what temperature do you ship reference standards?
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Can variants in the ctDNA Colorectal Cancer Panel (HD842) be detected with NGS?
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How are Horizon cfDNA Reference Standards different from other circulating tumor DNA controls?
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How are Horizon cfDNA Reference Standards fragmented?
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How can I open the NGS data .gz files?
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How do you manufacture your Horizon Reference Standards?
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How was the batch-specific supplementary NGS data produced and analysed?
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Is it possible to order custom versions of Horizon Reference Standard?
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Is the cell line background of the ctDNA Colorectal Cancer Panel (HD842) the same as that of the negative control (HD843)?
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What are cfDNA Reference Standards?
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What batch-specific supplementary NGS data is provided?
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What is MRD?
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What is the difference between ctDNA and cfDNA?
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What is the difference between the variants on the CoA and those in the supplementary batch-specific NGS data?
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Where can I access batch-specific supplementary NGS data?
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Why do I see different allele frequencies or different variants in my NGS data when comparing to the data provided with the reference standard?
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Why is the ctDNA Colorectal Cancer Panel (HD842) offered at very low allele frequencies?
Related Products
The Colorectal Cancer ctDNA Panel reference standard negative control is well characterized, cell line-derived control material manufactured under ISO 9001:2015 and ISO 13485:2016 that is tested negative for 10 variants across 10 genes clinically relevant to colorectal cancers.