Edit-R™ inducible lentiviral Cas9 nuclease gives you increased flexibility in your experiment
One of the most important components in any gene editing experiment is the Cas9 nuclease. This enzyme is responsible for generating the cut in the dsDNA that allows either NHEJ or HDR to occur and results in the experimentally induced change in the gene DNA sequence. However, controlling the timing of the experiment can be an important, even critical, part of the overall success or failure of gene editing. This can be especially true when trying to set up a pooled screen, generate a stable cell line without risk of increased metabolic load, or in cases where it is desirable to integrate the Cas9 in a precursor state and perform an experiment in a later derived cell state.
New inducible vector
Dharmacon Edit-R Inducible Lentiviral Cas9 Nuclease provides the temporal control necessary to ensure editing only occurs when it is required. Utilizing the tight, highly responsive Tet-On® 3G doxycycline inducible promoter and built on the platform of our existing human codon optimized constitutive Cas9 nuclease vectors, this new vector provides robust expression of the Cas9 nuclease when induced and minimal leak in the off state. Couple this control with the precise editing of Edit-R lentiviral and synthetic guide RNAs to result in an unparalleled level of options for your next CRISPR-Cas9 gene editing experiment. Available as either ready-to-use purified high-titer lentiviral particles or a purified plasmid format ready for packaging in the lab, this new vector will give you the precision you require.
- Learn more about CRISPR-Cas9 gene editing and the Dharmacon Edit-R platform
- Read about your options and select the right Cas9 nuclease expression vector and format for your experiment.
- Select from arrayed synthetic crRNA or pooled lentiviral sgRNA screening libraries
- Design and build your own custom synthetic crRNAs and lentiviral sgRNAs for your unique gene editing experiment