Learn about methods for CRISPR activation and determine the best path for your preferred cell model and experimental parameters.
In this blog, we will look at what to do once your gene editing experiment is finished and the different ways you will be able to characterize the successfully modified cell lines.
The presence of off-targets effects can greatly complicate the interpretation of experimental data. Dharmacon™ ON-TARGETplus™ siRNAs and CRISPRmod CRISPRi system are designed to knock down the target gene with minimal off-target effects and are powerful tools for orthogonal validation strategies resulting in highly specific readouts.
Our new Dharmacon™ ASO ordering tool is an online platform created for immediate price quoting and ordering of antisense oligonucleotides. With this custom DNA synthesis tool, you are able to create ASOs with a variety of chemical modifications at a scale and purity to meet your customized needs.
In this blog, we take a look at ClaretBio’s app note on using Horizon’s cfDNA reference standards in the development of their novel sample prep kit.
Learn about the MLR assay and why it is an essential component of the compound screening pipeline for drug development.
Describes methods of testing your cell culture for retroviral recombination.
CRISPR gene editing and siRNA gene silencing require the efficient delivery of the necessary reagents. This is easily achieved by optimizing delivery conditions. In this blog post we discuss techniques for optimization of a reverse transfection conditions and how to set up a 96 well plate to test 30 transfection conditions.
Learn how to examine your NGS data to investigate false positives and negatives, and potential downstream applications
Learn how to use Dharmacon™ Edit-R™ CRISPR reagents to make functional knockout models in human iPSCs.