Thanks to next-generation sequencing (NGS), we are starting to understand the mutational changes that occur across the board in the cancer genome. With this knowledge comes potential - novel mutated genes and the proteins that they encode are candidates for prognostic markers and/or new drug targets.
If a cell line tolerates being single cell diluted then plating 96-well plates at 1 cell per well in standard cell culture media is appropriate.
Cell lines remain one of the most important research tools in many labs today, be it for the study of basic subcellular processes or disease biology. The cell line selection is therefore a critical first step in any research project. Here’s a number of factors to consider, as well as resources to refer to when choosing your cell line (or lines).