A look at how a new method dubbed SHERLOCK adapts CRISPR-based technology to a diagnostic platform to allow mutation detections in DNA and RNA.
This blog explores how a selection of HAP1 knockout cell lines were used to confirm the involvement of DDR pathways in compounds mode of action, demonstrating opportunities for the exploitation of synthetic lethal interactions.
We explore the different ways to visualize proteins and check their expression. We will highlight in this blog the limits and drawbacks to their use.
Review of a paper published in Nature .com, detailing analysis of CRISPR-Cas9 sensitivity (Drop-out) screening, which resulted in the development of a streamlined screening pipeline to evaluate fundamental aspects of functional genomic screening.
The scientists at Horizon Discovery have published a robust and precise approach to generating translocations. This advancement facilitates the generation of relevant cell line models for oncology research.
Next-generation sequencing (NGS), has become a universal tool in diverse industries; most recently moving into the clinic for patient diagnosis. However, if labs wish to analyze patient-derived materials they must first face a hurdles labs to optimize and validate their workflow including determining the exact test limitations.
The high-throughput and increasingly affordable nature of next-generation sequencing (NGS) has led to its expanded use in routine clinical procedures. The relative simplicity of targeted enrichment cancer panels (available from a number of commercial providers) allows routine laboratories to simultaneously analyze the coding (exonic) regions of multiple cancer-related key genes. Combine this with the statistic diagnostic testing now influencing over 70 percent of all health care decisions1, the setting up or transitioning to NGS-based oncology panels for labs has never been more important.
A complete list of all our most frequently asked questions relating to HAP1 knockout cell lines. If you want to know how they're generated, how they're validated or how to find out if they're right for you - this is a great place to start.