Much information about the role of specific genes in fundamental biological processes and the onset and progression of genetic disease has been gleaned by researchers having the ability to selectively alter the genomic composition of individual genes and study the consequences. This approach enables researchers to observe the effects of a specific mutation, SNP or deletion in combination with the added layers of regulation present within the cell, including post-translational modification, epigenetic changes associated with chromatin structure, and transcriptional mechanisms.
Following transfection of CRISPR reagents, cells will need to be single cell diluted to obtain a clonal population. In this blog, we review the method used in-house.
It’s hard to keep up with the rapidly expanding world of CRISPR, and it’s starting to feel like CRISPR screens are being published every week, taking the technique from the cutting edge to the mainstream. If you’d like to understand a bit more about CRISPR screens, here’s a number of fantastic publications that have really moved this technology forward.
A review of the main 5 tips to choose and use a cell line. We highlight the importance of checking for authenticity, and contamination.
Get a head start when planning your next RNA interference experiments with our most popular advice.
Experimental results from a comparison of ON-TARGETplus siRNA and CRISPRi reagents reveals both technologies exhibit high specificity. This post also demonstrates the using the two in conjunction to produce a robust strategy for orthogonal hit validation.