RNAi Controls

Critical to any gene silencing or microRNA modulation experiment, RNAi controls enable accurate interpretation for reliable, reproducible results.

Recommended controls for use in RNAi experiments

  • Positive control Validated RNAi reagents to optimize and monitor efficiency of delivery across experimental replicates, conditions or cell types.
  • Non-targeting negative control An RNAi reagent with a non-targeting sequence to provide a baseline reference for target-specific knockdown and distinguish sequence-specific silencing from non-specific effects.
  • RISC-Free™, Empty Vector or mock transfection A specialized control or experimental condition that lacks a fully functional RNAi component to detect non-RNAi cellular effects (toxicity or gene expression changes) caused by the transfection or transduction.
  • Fluorescent Controls Fluorescent dye-labeled controls, including novel nuclear-localizing transfection indicators permit correlation of signal intensity with optimal siRNA/microRNA transfection conditions.

siRNA Controls

  • ON-TARGETplus Controls

    Validated positive controls and non-targeting negative controls utilizing patented modifications for optimal specificity. Available as pools or single siRNAs.

  • Accell Controls

    Effectively optimize Accell siRNA delivery conditions with fluorescent or unlabeled positive and negative controls. Kits of validated controls available.

  • siGENOME Controls

    A robust selection of validated positive, negative, and novel RISC-Free® controls to effectively assess all aspects of RNAi experiments.

  • Lincode Controls

    Endogenous lncRNA positive control and non-targeting control siRNA and pool reagents for high-confidence experiments.

  • siGLO Controls

    Fluorescent controls are effective tools for visualizing transfection success. Choose from positive control siRNAs or our novel transfection indicators.

  • Reporter Genes, siSTABLE and other Controls

    Choose from siSTABLE-modified controls, siRNA for silencing reporter genes (GFP, Luciferase, etc.) or other experimental needs.

shRNA Controls

microRNA Controls

Accell Control siRNA Kits

A tool set to assess and optimize Accell technology in your cell type, and to ensure effective controls during initial gene silencing experiments with Accell siRNA reagents.

Each kit contains:

  • Accell GAPD Control siRNA (Human, Mouse, or Rat)
  • Accell Cyclophilin B Control siRNA (Human, Mouse, or Rat)
  • Accell Non-targeting siRNA #1
  • Choice of Green or Red Accell Non-targeting siRNA
  • 5X Buffer
  • Accell siRNA Delivery Media
Species
Accell Control siRNA Kits (Green) Human, Mouse, Rat
Accell Control siRNA Kits (Red) Human, Mouse, Rat

siGENOME Control Kits

A comprehensive array of siRNA control reagents for use in any siRNA experiment.

Each siGENOME Controls Basic Kit contains 5nmol each of:

  • siGENOME Lamin A/C Control siRNA (Human, Mouse, or Rat)
  • siGENOME Cyclophilin B Control siRNA (Human, Mouse, or Rat)
  • siGENOME Non-targeting siRNA #2
  • siGENOME Non-targeting siRNA #5
  • siGENOME RISC-Free Control siRNA
  • siGLO Green Transfection Indicator
  • siGENOME Non-targeting siRNA Pool #2

Each siGENOME Controls Complete Kit additionally contains:

  • Three additional siGLO control siRNAs
  • TOX Transfection Control
Species
siGENOME Controls Basic Kit Human, Mouse, Rat
siGENOME Controls Complete Kit Human, Mouse, Rat

Which RNAi control is best for you?

Use the guide below to help determine which RNAi control is best for your research needs.

Product Line Endogenous Positive Control Exogenous Positive Control Negative Control Fluorescent Label Control Kits
siRNA siGenome siRNA Lamin A/C, PPIB, GAPD Non-targeting, RISC-Free
ON-TARGETplussiRNA PPIB, GAPD Non-targeting
Accell siRNA PPIB, GAPD eGFP Non-targeting
siSTABLE siRNA PPIB, GAPD Non-targeting
siGLO siRNA Lamin A/C, PPIB RISC-Free
siGLO Transfection Indicators
Reporter gene controls RLuc,FLuc,GPF,b-glal, GL2 and GL3 Luciferase
Lincode siRNA GAS5 Non-targeting
shRNA SMARTvector Lentiviral shRNA PPIB, GAPD Luciferase Non-targeting, empty vector
SMARTvector Inducible shRNA PPIB, GAPD Non-targeting
GIPZ Lentiviral shRNA EG5,GAPD Non-targeting, empty vector
TRIPZ Inducible Lentiviral shRNA GAPD Non-targeting, empty vector
TRC Lentiviral shRNA eGFP Empty vector
microRNA shMIMIC Lentiviral microRNA PPIB, GAPD Non-targeting, empty vector
shMIMIC Inducible microRNA PPIB, GAPD on-targeting
miRIDIAN microRNA Mimics miR-122,3' UTR controls Negative Control
miRIDIAN microRNA Hairpin Inhibitors miR-16 Negative Control