Use the expertise of our bioinformatics team to help with your NGS and screening analysis
71 result(s)
Reverse transfection for high-throughput CRISPR studies
Choosing the right cell-based screen from the plethora of options available can quickly become a complicated decision process. Here, we review two of the major options: cell panel and functional genomic screening.
Considerations for design of an optimal ssDNA donor for CRISPR-Cas9-mediated precise gene editing
Optimal guide RNAs balance functionality and specificity
With so many to choose from - how do you know which Cas9 is best for your experiment?
Every year since 2007, The Scientist Magazine gets hundreds (thousands?) of nominations for their Top 10 Innovations Award.
Now SMARTCas9 gives you a CHOICE of promoters for optimal Cas9 expression in your specific cells.
Ordering guidelines for quantities of crRNA and tracrRNA
After all the hard work of editing your cell line, you want to have confidence in your new research model. So, how do you verify your cell line is what you expect it to be? Could a heterogeneous cell population be obscuring your editing effects? Is observed phenotype being caused by the targeted gene edit, or unintended off-target effects? Here we discus ways to add supporting data to validate your gene-engineering projects.