Functional genomic screening allows for the elucidation of the mechanism of action of exogenous agents which modulate intracellular gene function such as pharmaceutical agents and other compounds. Read about the various considerations, approaches and interpretations when conducting a screen to investigate novel compounds and analyzing the screening data.
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With the requirement of thorough in vitro assays and high throughput screening technologies to ensure drug safety and efficacy, make sure you're fully ready for when you submit your IND application by assessing immunogenicity with an MLR assay
In pooled CRISPR drug-gene screens two selection mechanisms can be applied, positive or resistance and negative or sensitivity screening, with each having various considerations and uses
Functional Genomic and Cell Panel Screening can individually provide comprehensive data sets to support therapeutic development, but when utilized in combination they identify novel targets which are cross-validated by independent approaches.
Learn about how T cell exhaustion poses a significant challenge in immuno-oncology and the way epigenetic mechanisms can help develop strategies for reinvigorating exhausted T cells.
Learn about the MLR assay and why it is an essential component of the compound screening pipeline for drug development.
Large-scale gain-of-function screens have not yet shown the same popularity as loss-of function experiments, mainly due to logistical complications of using the traditional reagents (ORFs). Here we explain the hurdles to setting up a high-throughput ORF screen, and how CRISPRa offers a new solution that can expand the screening toolbox to maximize your window of discovery.
Learn about Horizon's CRISPRsc (CRISPR single cell) platform that combines the flexibility of pooled screening with the power of transcriptomics at single cell resolution.
A review of two studies demonstrating that large-scale pooled base editor screens can separate pathogenic from benign mutations and identify variants that are associated with altered cellular growth or response to DNA-damaging agents.
A summary of a recent publication in The Journal of Biotechniques showing the utility of Horizon's synthetic sgRNA for CRISPR mediated transcriptional activation studies.