A blog article and application note demonstrating the use of Revvity's Pin-point base editing platform with sensitive cell types
The Toll-like receptors (TLRs) are a family of evolutionarily ancient Patten Recognition Receptors (PRRs) that rapidly detect microbial infection and stimulate the production of pro-inflammatory and antiviral cytokines and chemokines, as well as initiating significant metabolic shifts within the cell. Acting as cellular sentinels for infectious threats, signalling downstream of TLRs sets the stage for successful development of adaptive immunity 1.
The adherens junction (AJ), with the core cadherin-catenin complex, is an integral system of cell-cell adhesion. The AJ works to anchor the actin cytoskeleton intracellularly to the plasma membrane while simultaneously linking individual cells through extracellular interactions.
The reasoning and research behind our Dharmacon™ CRISPRi platform recently published in The CRISPR Journal.
Knowing the distinction between "synthesis scale" and "yield" is critical when ordering custom oligos. Here we explain the terms, so you can make sure to get the right amount for your needs.
All you need to know if you're thinking about labeling your custom oligos with fluorophores.
On Wednesday October 19, The Medical Device Innovation Consortium (MDIC) announced its partnership with Horizon Discovery to develop and manufacture somatic reference samples (SRSs) to simplify and support the validation of NGS-based cancer diagnostics.
Learn about methods for CRISPR activation and determine the best path for your preferred cell model and experimental parameters.
In this blog, we will look at what to do once your gene editing experiment is finished and the different ways you will be able to characterize the successfully modified cell lines.
The presence of off-targets effects can greatly complicate the interpretation of experimental data. Dharmacon™ ON-TARGETplus™ siRNAs and CRISPRmod CRISPRi system are designed to knock down the target gene with minimal off-target effects and are powerful tools for orthogonal validation strategies resulting in highly specific readouts.