In pooled CRISPR drug-gene screens two selection mechanisms can be applied, positive or resistance and negative or sensitivity screening, with each having various considerations and uses
Functional Genomic and Cell Panel Screening can individually provide comprehensive data sets to support therapeutic development, but when utilized in combination they identify novel targets which are cross-validated by independent approaches.
When making a knockout cell line using CRISPR, western blotting is a useful tool to help in the verification of the cell line. However, correct interpretation of results is crucial. Here we summarize the possible fates of a protein after knockout and explore how best to validate a knockout cell line
Learn about how T cell exhaustion poses a significant challenge in immuno-oncology and the way epigenetic mechanisms can help develop strategies for reinvigorating exhausted T cells.
A study where we have employed orthogonal target validation strategies with 3 orthogonal LOF technologies (CRISPRko, CRISPRi, and siRNA) with genes involved in maintenance of the mesenchymal-to-epithelial transition (MET).
Learn about considerations around the development of the Dharmacon™ Edit-R™ CRISPRko algorithm for functional and specific guide RNA
Cell panel screening assays are an essential part of the drug discovery toolbox. However, fully resolving the drug response profiles of slow-acting therapeutics remains challenging with conventional short term assay formats. Elucidation of the effects of these drug candidates, and those that target epigenetic pathways, requires much greater optimization of longer-term assay conditions.
Reliable and consistent quality reagents are critical to experimental success and reproducibility. Learn about how Dharmacon RNA and DNA oligonucleotide synthesis delivers that level of quality, and explore troubleshooting tips for investigating unexpected results in your experiments.
The reasoning and research behind our Dharmacon™ CRISPRi platform recently published in The CRISPR Journal.
Knowing the distinction between "synthesis scale" and "yield" is critical when ordering custom oligos. Here we explain the terms, so you can make sure to get the right amount for your needs.