A blog article and application note demonstrating the use of Revvity's Pin-point base editing platform with sensitive cell types
The Toll-like receptors (TLRs) are a family of evolutionarily ancient Patten Recognition Receptors (PRRs) that rapidly detect microbial infection and stimulate the production of pro-inflammatory and antiviral cytokines and chemokines, as well as initiating significant metabolic shifts within the cell. Acting as cellular sentinels for infectious threats, signalling downstream of TLRs sets the stage for successful development of adaptive immunity 1.
The adherens junction (AJ), with the core cadherin-catenin complex, is an integral system of cell-cell adhesion. The AJ works to anchor the actin cytoskeleton intracellularly to the plasma membrane while simultaneously linking individual cells through extracellular interactions.
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Revvity molecular reference standards are available for both germline and somatic variants. In this article we cover why this distinction is important, and what it means for the allele frequencies of variants in these two types of controls.
Functional genomic screening allows for the elucidation of the mechanism of action of exogenous agents which modulate intracellular gene function such as pharmaceutical agents and other compounds. Read about the various considerations, approaches and interpretations when conducting a screen to investigate novel compounds and analyzing the screening data.
With the requirement of thorough in vitro assays and high throughput screening technologies to ensure drug safety and efficacy, make sure you're fully ready for when you submit your IND application by assessing immunogenicity with an MLR assay
In pooled CRISPR drug-gene screens two selection mechanisms can be applied, positive or resistance and negative or sensitivity screening, with each having various considerations and uses
Functional Genomic and Cell Panel Screening can individually provide comprehensive data sets to support therapeutic development, but when utilized in combination they identify novel targets which are cross-validated by independent approaches.
When making a knockout cell line using CRISPR, western blotting is a useful tool to help in the verification of the cell line. However, correct interpretation of results is crucial. Here we summarize the possible fates of a protein after knockout and explore how best to validate a knockout cell line