Design and order libraries of vector-based reagents

  • Our online tool offers the flexibility to select clones and generate a library for your unique experimental needs
  • Choose vector-based reagents from our algorithm-optimized, predesigned lentiviral product lines of shRNA by Dharmacon™ or from popular mammalian cDNA or ORF gene-expression collections; formatted as glycerol stocks.
  • For synthetic reagents, please see instructions for designing synthetic Cherry-pick libraries
  • Select specific clones for a gene or add everything available
  • Clones will automatically be separated onto different plates by antibiotic resistance marker and reagent format

Build a library now

When Horizon shRNA, or over-expression libraries do not match your experimental requirements, you can make your own custom clone library with ease, speed and economy.

You can cherry-pick from our glycerol stock format shRNA and mammalian over-expression reagents for human and mouse reagents to create the ideal clone collection for your particular needs.

Here's how:
  1. Load gene identifiers for your desired reagents
    • NCBI Gene ID or Gene Symbol
    • Library reagent catalog numbers
  2. Select the reagent type
  3. Review the results for accuracy and reagent availability
  4. Select the reagents and reagent formats to include on the plate
  5. Add to cart and check out!
Cherry-pick Library options
Use the Library Plater to order these reagents in 96-well plates
shRNA available GIPZ, TRIPZ, TRC
Over-expression reagents Mammalian Genome Collection (MGC), Precision LentiORFs, CCSB-Broad Lentiviral Expression Library, ORFeome Collaboration Collection, Human ORFeome v8.1 Library, CCSB Human ORFeome
Format Glycerol stock
Species Human or mouse
Plate type 96 well; 20 well minimum of same reagents
Which shRNA reagent is right for you?

Horizon offers three complete predesigned reagents across human and mouse genomes. Use the table below to help you in determining the right shRNA reagent for your needs.

Species Human, mouse Human Human, mouse
Promoter Human CMV (Pol II) TRE min-CMV U6 (Pol II)
Hairpin format microRNA-adapted microRNA-adapted Simple
Fluorescent reporter TurboGFP TurboRFP None
Inducible expression No Yes No
Which over-expression reagent is right for you?

Horizon offers five different mammalian open reading frame (ORF) collections and the Mammalian Genome Collection (MGC) cDNA collection. Use the table below to help you in determining the right over-expression reagent for your needs.

Mammalian Genome Collection (MGC) Precision LentiORF CCSB-Broad Lentiviral Expression ORF ORFeome Collaboration Collection Human ORFeome V8.1 CCSB Human ORFeome
Species Human, Mouse Human Human Human, Mouse Human Human
Fully Sequenced Yes Yes Yes Yes Yes No
Expression ready Some Yes Yes No No No
Subcloning method Restriction digest N/A N/A Gateway® Gateway® Gateway®
Selection marker N/A GFP V5 epitope tag N/A N/A N/A

All reagents are delivered as arrayed glycerol stocks in 96-well plates

  • shRNA clones
    Select from either: GIPZ, TRIPZ, or TRC lentiviral shRNA collections; minimum 20 constructs per order. We recommend selecting 3-5 constructs per gene.
  • Over-expression clones
    MGC collection: Cannot be mixed with other collections, minimum 20 constructs per order; clones will be plated according to antibiotic requirements
  • ORF collections
    May be combined with other ORF collections to increase coverage for desired library; minimum 20 constructs from a single collection per order. Clones will be plated by collection. (e.g. a customer library of 20 ORFeome collaboration clones and 5 hORFeome v8.1 clones will qualify for library plating, a custom library of 15 ORFeome collaboration clones and 10 hORFeome v8.1 will not)

Lentiviral guide RNA

Investigation of entire gene families or biological pathways is enabled by our broad suite of custom and pre-defined libraries of CRISPR-Cas9 reagents that take advantage of our algorithm-optimized guide RNA designs for high functionality and superior specificity.

microRNA-adapted shRNAs

Designed using microRNA scaffold-specific attributes for highly efficient processing via the endogenous RNAi pathway while minimizing off-target potential

  • SMARTvector Lentiviral shRNA
    Advanced rationally-designed microRNA-based shRNA for human, mouse, and rat genes. Choose from seven constitutive promoters and three reporter options to tailor SMARTvector shRNA for specific cells.
  • SMARTvector Inducible Lentiviral shRNA
    All the benefits of SMARTvector lentiviral shRNAs combined with latest generation of powerful Tet-inducible technology for unprecedented control over gene silencing and reduced off-targeting.
  • GIPZ Lentiviral shRNA
    Efficient gene silencing with a microRNA-adapted shRNA design. Available as lentiviral vector constructs or high-titer lentiviral particles for human and mouse
  • TRIPZ Inducible Lentiviral shRNA
    Inducible shRNA expression allows for tightly regulatable RNAi experiments. Available as lentiviral vector constructs for human.
  • SMARTvector Lentiviral shRNA Pooled Libraries
    High-titer pooled screening libraries of constitutive shRNAs for pre-defined gene libraries in human, mouse, and rat
  • SMARTvector Inducible Lentiviral shRNA Pooled Libraries
    High-titer pooled screening libraries of inducible shRNAs for pre-defined gene libraries in human, mouse, and rat. 

Simple hairpin shRNAs

Offers a classic hairpin, rules-based shRNA design utilizing a simple hairpin of 21 base pair sense and antisense stem, and a 6 base pair loop

  • TRC Lentiviral shRNA
    Lentiviral shRNA collection from The RNAi Consortium (TRC) with coverage in human and mouse.

cDNA and ORF libraries

Pre-defined gene family collections are available for many of our cDNA and ORF reagents. Choose the reagent that best fits your experimental needs.

  • Mammalian Genome Collection
    The mouse and human MGC libraries contain popular gene families in expression-ready and other vector backbones.
  • Precision LentiORFs
    Expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.
  • CCSB-Broad Lentiviral Expression Library
    Expression-ready human ORFs in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.
  • ORFeome Collaboration Libraries
    Sequence-verified human and mouse open reading frames (ORFs) subcloned into Gateway® recombinational entry vectors.
  • Human ORFeome V8.1 Library
    Fully-sequenced collection of Gateway-adapted ORFs representing the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.
  • CCSB Human ORFeome Libraries
    The Gateway® adapted human Entry ORF libraries, ideal for easily moving entire families of ORFs into your choice of protein expression systems.