Knockout and tagged gene-editing can create cell lines ideal for antibody validation. Using CRISPR CAS technology, Horizon developed a streamlined process to cut production time. Read more in our interview with Lead Scientist Daniel Lackner.
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The scientists at Horizon Discovery have published a robust and precise approach to generating translocations. This advancement facilitates the generation of relevant cell line models for oncology research.
There exist now a range of techniques to perform genome editing, such as ZFN, CRISPR, TALENS and AAV, each with their own strengths and weaknesses. However, one consistent element that has a significant impact on the success of that editing event when generating an isogenic cell line is the choice of parental cell line to be engineered.
Here you'll find a complete list of all our most frequently asked questions relating to HAP1 knockout cell lines. If you want to know how they're generated, how they're validated or how to find out if they're right for you - this is a great place to start.
If you've followed our guide to planning a successful genome editing experiment, then you'll hopefully be working in optimal conditions, and have a good idea of your guide's editing efficiency. This number should give you a rough idea of how many clones you're going to have to screen to find a targeted clones.
Reporter gene assays are widely used to study the regulation of gene expression. We have developed a suite of endogenous reporter cell lines which measure natural levels of protein expression and promoter activity. By measuring at the endogenous level, this system provides an advantage over other technologies which use exogenous plasmid-based overexpression systems.
Much information about the role of specific genes in fundamental biological processes and the onset and progression of genetic disease has been gleaned by researchers having the ability to selectively alter the genomic composition of individual genes and study the consequences. This approach enables researchers to observe the effects of a specific mutation, SNP or deletion in combination with the added layers of regulation present within the cell, including post-translational modification, epigenetic changes associated with chromatin structure, and transcriptional mechanisms.
If a cell line tolerates being single cell diluted then plating 96-well plates at 1 cell per well in standard cell culture media is appropriate.
Cell lines remain one of the most important research tools in many labs today, be it for the study of basic subcellular processes or disease biology. The cell line selection is therefore a critical first step in any research project. Here’s a number of factors to consider, as well as resources to refer to when choosing your cell line (or lines).
Learn about Horizon's assay and screening capabilities in primary immune cell types such as regulatory T cells (Tregs), myeloid derived suppressor cells (MDSCs) and regulatory B cells (Bregs).