Open Reading Frames (ORFs) are useful tools for gene overexpression studies, RNAi rescue experiments, and protein localization or structural analysis studies. ORFs have both 5' and 3' UTRs removed, thereby bypassing the native regulation mechanisms for a shortcut to protein expression.

Precision LentiORFs

Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

CCSB Human ORFeome Collection

Gateway® adapted human entry ORFs are ideal for easily moving entire families of ORFs into your choice of protein expression systems.

Cherry-pick library tool

Design your own custom cDNA or ORF libraries

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CCSB-Broad Lentiviral Expression Collection

Human ORFS in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

ORFeome Collaboration Clones

ORFeome Collaboration clones are sequence-verified, human open reading frames (ORFs) subcloned into Gateway® recombinational entry vectors.

hORFeome v8.1 Collection

This fully-sequenced collection of Gateway-adapted ORFs represents the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

Non-mamalian cDNAs and ORFs

Overexpress genes from Yeast, C. elegans, E. coli, Zebrafish or Xenopus laevis.

Precision lentiORF

RNAi Rescue using Precision LentiORF

The Precision LentiORF collection provides a comprehensive set of ready-to-use clones that permit RNAi rescue in studies that utilize 3'-UTR siRNA reagents. This study highlights the necessary controls and optimization required for an RNAi rescue experiment and demonstrates the utility of these constructs when validating phenotypes associated with siRNA-mediated silencing.

Top five considerations for ORF selection

Five tips to quickly and effortlessly select the right clone format for your over-expression project.

Using ORFs with variable stop positions

Many Precision LentiORF clones are available either with or without the native stop codon.