Drug development with nuclear receptor knockout and humanized rat models
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of Phase I (Cytochrome P450s), Phase II drug metabolizing enzymes and drug transporter genes in response to stimulation from xenobiotics including prescription drugs.
Exploring inducible systems to inactivate wildtypes
This webinar will describe the level of knockdown that can be achieved with degron-tagged CRISPR interference proteins, how best to deploy dox-inducible Cas9, and various approaches to delete wild-type alleles of genes so as to replace them with inactive alleles that could be applied at scale.
The Effect of Formalin on Clinical Diagnostics
The use of formalin fixative stems from its ability to cross-link proteins, however it has also been reported to induce methylol modifications and acid-mediated hydrolysis which can confound genotyping analysis. Similarly, variability in the fixation time, delay of fixation and type of fixation effect tumor biopsies and subsequent sequencing.
Understanding and Controlling for Sample and Platform Biases in NGS Assays
In this webinar we will focus on next generation sequencing and discuss: the effect of formalin treatment on molecular protocols and how it can be controlled, how to select the sequencing platform most appropriate for your sample, evaluation of whether structural variants are being detected by your assay and how Reference Standards can be used in each validation parameter.