Here we discuss a recent paper by Gisela Jimenez-Duran and colleagues highlighting how a whole genome CRISPR knockout screen, carried out by Horizon Discovery, has enabled identification of a pre-existing inhibitor that can regulate pro-inflammatory macrophages.
Thinking about doing CRISPR screening for knock out, CRISPR activation or CRISPRi applications? Unsure whether a synthetic arrayed or pooled lentiviral screening library is best for your project? We are here to help.
A roundtable discussion with several of the women scientists at Horizon Discovery's Boulder office sharing personal reflections after Jennifer Doudna along with Emmanuelle Charpentier won the Nobel Prize in Chemistry this year.
This article briefly describes how our reference standards are produced and the quality control measures to ensure the standards are reliable from batch to batch.
Here are some essential points to think about when ordering your oligos for in vivo experiments.
A summary of a recent publication in The CRISPR Journal showing that Horizon's integrated pooled CRISPRko and high throughput cell panel screening platform together offer clinically relevant information for therapeutic targets.
Here we talk about the role of the Edit-R Pooled sgRNA Indexing PCR Primers and answer the most common questions about the Indexing PCR and Sequencing Primer kit.
Demonstration of a repeatable pooled lentiviral sgRNA screen in primary human T cells
Gene editing by CRISPR has led to significant advances in immunotherapy development. Here we offer several tips for successful editing in T cells, including recommendations for cell handling, nucleofection, and analysis.
Read about the top 4 considerations when deciding between clones and pools