If you've followed our guide to planning a successful genome editing experiment, then you'll hopefully be working in optimal conditions, and have a good idea of your guide's editing efficiency. This number should give you a rough idea of how many clones you're going to have to screen to find a targeted clones.
A proof-of-concept study where CRISPR-Cas9 reagents and guides to 3 genes were added to the same cell population and then a representative subset of clonal cell lines were analyzed for multiplexed gene editing efficiency.
A discussion of novel methodology and research in primary human monocytes recently published by the labs of Dr. Alex Marson and Dr. Nevan Krogan.
Detecting proteins with confidence starts with antibody validation. Here, we've outlined the key points.
Review of publications using HAP1 cells to set up validation assays beyond the typical western blot.
Our top 5 tips for working with HAP1 cells.
Here we discuss a recent paper by Gisela Jimenez-Duran and colleagues highlighting how a whole genome CRISPR knockout screen, carried out by Horizon Discovery, has enabled identification of a pre-existing inhibitor that can regulate pro-inflammatory macrophages.
Demonstration of a repeatable pooled lentiviral sgRNA screen in primary human T cells
Read about the top 4 considerations when deciding between clones and pools
Technology from Horizon Discovery has been awarded Top Innovation of the Year by The Scientist Magazine for the past seven consecutive years.