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The presence of off-targets effects can greatly complicate the interpretation of experimental data. Dharmacon™ ON-TARGETplus™ siRNAs and CRISPRmod CRISPRi system are designed to knock down the target gene with minimal off-target effects and are powerful tools for orthogonal validation strategies resulting in highly specific readouts.
CRISPR gene editing and siRNA gene silencing require the efficient delivery of the necessary reagents. This is easily achieved by optimizing delivery conditions. In this blog post we discuss techniques for optimization of a reverse transfection conditions and how to set up a 96 well plate to test 30 transfection conditions.
Accell siRNA has been widely literature-cited for gene silencing in primary immune cells, which have historically been difficult to transfect. Learn more here.
Learn how ON-TARGETplus siRNA from Dharmacon ensures target specificity while avoiding off-target effects to give you confidence in your RNAi studies.
Want to optimize RNAi experiments in difficult-to-transfect cell lines? Read our workflow recommendations for optimal delivery and functional silencing with Accell siRNA.
Large-scale gain-of-function screens have not yet shown the same popularity as loss-of function experiments, mainly due to logistical complications of using the traditional reagents (ORFs). Here we explain the hurdles to setting up a high-throughput ORF screen, and how CRISPRa offers a new solution that can expand the screening toolbox to maximize your window of discovery.
Learn how to use our Dharmacon™ siDESIGN center for isoform or gene variant-specific siRNAs
Gene modulation is now more flexible and accessible thanks to Horizon's new CRISPRmod CRISPRi portfolio. The system leverages an inactive Cas9 complex to block transcription resulting in precision targeted gene down regulation. Read more about our newest offering:
A summary of a recent publication in The Journal of Biotechniques showing the utility of Horizon's synthetic sgRNA for CRISPR mediated transcriptional activation studies.