Guidelines for making the most out of your CRISPR experiment
17 result(s)
Gene editing by CRISPR has led to significant advances in immunotherapy development. Here we offer several tips for successful editing in T cells, including recommendations for cell handling, nucleofection, and analysis.
Read about the top 4 considerations when deciding between clones and pools
Examples of studies using siRNAs targeting lncRNAs to inspire your next experiment.
Isolation of genomic DNA from formalin fixed paraffin embedded (FFPE) tissues is a critical step for molecular diagnostic (MDx) assays. Here we investigate extraction of DNA from FFPE using five comparable methods.
Tips and tricks for RNAi success - make sure to include necessary controls in your transfections!
After all the hard work of editing your cell line, you want to have confidence in your new research model. So, how do you verify your cell line is what you expect it to be? Could a heterogeneous cell population be obscuring your editing effects? Is observed phenotype being caused by the targeted gene edit, or unintended off-target effects? Here we discus ways to add supporting data to validate your gene-engineering projects.
The only self-delivering siRNA for difficult-to-transfect cells carries a functional guarantee.
CRISPR technology now allows genes and molecular pathways to be examined with greater definition. We look at how knockout cell lines, either together with gene rescue and replication of disease mutations or as an independent cell model, can be used to validate your research and extend your findings
With new advances in technology, we're now moving more towards large panel next-generation sequencing (NGS) assays and whole exome sequencing (WES). NGS is gaining popularity thanks to high-throughput capability and reduction in cost per sample.