If you've followed our guide to planning a successful genome editing experiment, then you'll hopefully be working in optimal conditions, and have a good idea of your guide's editing efficiency. This number should give you a rough idea of how many clones you're going to have to screen to find a targeted clones.
Describes methods of testing your cell culture for retroviral recombination.
CRISPR gene editing and siRNA gene silencing require the efficient delivery of the necessary reagents. This is easily achieved by optimizing delivery conditions. In this blog post we discuss techniques for optimization of a reverse transfection conditions and how to set up a 96 well plate to test 30 transfection conditions.
Learn how to use Edit-R CRISPR reagents from Dharmacon to make functional knockout models in human iPSCs.
Guidelines for making the most out of your CRISPR experiment
Learn how precise control of Cas9 expression helps reveal the function of essential genes.
Once you have carried out your gene editing experiment, how will you monitor the result?
A proof-of-concept study where CRISPR-Cas9 reagents and guides to 3 genes were added to the same cell population and then a representative subset of clonal cell lines were analyzed for multiplexed gene editing efficiency.
Want to optimize RNAi experiments in difficult-to-transfect cell lines? Read our workflow recommendations for optimal delivery and functional silencing with Accell siRNA.
A discussion of novel methodology and research in primary human monocytes recently published by the labs of Dr. Alex Marson and Dr. Nevan Krogan.