edit-r

Edit-R All-in-one sgRNA + Cas9

All-in-one lentiviral sgRNA

• Combine Cas9 and sgRNA expression into a single vector for efficient gene knockout & unparalleled simplicity; available as glycerol stocks and high-titer purified particles

All-in-one lentiviral sgRNA positive controls and kits

• All-in-one lentiviral sgRNAs targeting well characterized genes help verify gene editing efficiencies.

All-in-one lentiviral sgRNA non-targeting controls

• All-in-one lentiviral sgRNAs designed and validated to not target any gene in human, mouse or rat genomes.

Edit-R synthetic guide RNA

Synthetic sgRNA

• Algorithm designed synthetic CRISPR single guide RNA for targeted gene knockout of any human or mouse gene.

Synthetic sgRNA positive controls

• Species-specific sgRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.

Synthetic sgRNA non-targeting controls

• Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific sgRNA.

Synthetic crRNA

• Guaranteed to edit your target! Algorithm-optimized crRNA for genome-wide coverage of human, mouse, or rat genes. Modifications for nuclease resistance improve DNA-free editing. Simply search for your gene!.

tracrRNA

• Edit-R trans-activating CRISPR RNA (tracrRNA) is synthetic, HPLC-purified, long RNA required for use with Edit-R crRNA to form the complex that programs Cas9 nuclease. It is modified for nuclease resistance and can be used with modified or unmodified Edit-R crRNA.

Synthetic crRNA positive controls and detection primers

• Species-specific crRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.

Synthetic crRNA non-targeting controls

• Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific crRNA.

Edit-R lentiviral guide RNA

Lentiviral sgRNA

• Algorithm-optimized sgRNA for genome-wide coverage of human, mouse, or rat genes. Provided as high-titer lentiviral particles and glycerol stocks.

Lentiviral sgRNA positive controls and detection primers

• Species-specific sgRNAs targeting well-characterized genes to determine the effectiveness of your gene editing conditions for maximal efficiency.

Lentiviral sgRNA non-targeting controls

• Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific sgRNA.

Edit-R Cas9 nucleases

Cas9 nuclease mRNA

• Purified Cas9 mRNA for transient Cas9 nuclease expression.

Cas9 nuclease protein NLS

• Purified Cas9 protein ready to use for DNA-free nuclease expression.

Lentiviral Cas9 nuclease reagents

• Purified lentiviral particles or plasmid DNA for generation of stable Cas9 nuclease-expressing cell populations. Constitutive or inducible promoter options are available. 

Edit-R knockout screening libraries

Cherry-pick libraries

• Have a favorite gene list? Customize and order plates of predesigned crRNA for knockout studies in your targets of interest. 

Pooled lentiviral sgRNA libraries

• High-titer pooled screening libraries for pre-defined gene sets in human and mouse.

Edit-R custom guide RNA design

CRISPR Design Tool

• Use the Edit-R algorithm to design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA for 30+ species or alternative nucleases.

Edit-R HDR donor templates

HDR Donor Designer - oligo

• Design and order a single-strand DNA donor (≤ 150 nt) for insertion, deletion, or other alteration.

HDR Donor Designer - plasmid

• Design and order a plasmid DNA donor kit for insertion of an mKate2 or EGFP fluorescent marker, or a custom insert

HDR plasmid donor kits

• Rapidly and easily assemble a plasmid donor for HDR

HDR plasmid donor components

• Quickly and efficiently build a HDR donor plasmid

HDR plasmid donor primers

• PCR components for Edit-R Plasmid Donor Kits

CRISPR-Cas9 Gene Editing Applications

Synthetic sgRNA for CRISPR-Cas9 Experiments

• CRISPR genome knockout with a synthetic 99-mer single guide RNA

CRISPR guide RNA functionality

• Development of an algorithm for functional knockout, not just cutting

CRISPR guide RNA specificity

• Rigorous alignment tools ensure high specificity of gene editing

Inducible lentiviral Cas9 nuclease

• An inducible lentiviral Cas9 nuclease for increased experimental flexibility

Homology-directed repair with a DNA donor oligo

• Considerations for successful HDR experimental design

Homology-directed repair (HDR) with a plasmid donor

• Considerations for successful HDR experimental design 

100% of Edit-R predesigned guide RNA are guaranteed to edit!

All Edit-R predesigned guide RNA's are guaranteed to edit your target, or we will replace it! No restrictions on Cas9 nuclease formats – if your Edit-R positive control works, so will your gene-specific guide RNA.

The Edit-R guide RNA guarantee

We guarantee that EVERY predesigned synthetic crRNA, synthetic sgRNA, lentiviral sgRNA and All-in-one lentiviral sgRNA will provide successful editing at the target site when delivered as described in the Edit-R Technical Manuals.

The Edit-R guide RNA guarantee is valid when used with any wild type S. pyogenes Cas9 nuclease, including mRNA, expression plasmid, protein, or stable Cas9 expression, and Edit-R crRNAs must be used with Edit-R tracrRNA for the guarantee to apply.

Analysis of editing of the treated cell population must be shown using a T7EI or Surveyor mismatch detection assay. If successful editing is not observed for a predesigned Edit-R guide RNA while an appropriate side-by-side Edit-R positive control is successful, a one-time replacement of a different predesigned Edit-R guide RNA of the same format and quantity will be provided at no cost.

A replacement will only be approved upon discussion with our Scientific Support team.

Successful editing at the DNA level does not always lead to functional gene knockout; it is recommended to test multiple guide RNAs to determine the most effective guide RNA for knockout of your target gene.

This guarantee does not extend to any accompanying experimental costs, does not apply to guide RNAs ordered via the CRISPR Design Tool, and will not be extended to the replacement guide RNA.