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Cell line engineering services

Delivering exceptional value across the drug discovery pipeline

Trusted expertise and proven technology

Creating your own CRISPR-Cas9 edited custom cell line model can require significant investment in both lab and human resources. Horizon has the experience and expertise to offer an affordable solution, allowing our partners to focus on the most critical aspect of any research project - delivering accurate data and publishing reliable results.

Our expertise comes from thousands of successful gene editing projects across many cell types – including challenging cell lines such as iPSCs and primary cells.

Design your cell line

Use our intelligent cell line configurator tool to build a cell line based on YOUR specific project needs! Simply enter your modification type, gene, and cell line specifics to receive your quote instantly.

Capabilities

Horizon offers a wide range of cell line engineering services:

 Knockouts

 Knock-ins

Unique projects

Standard knockouts
  • Starting at £7,500/$9,950/€8,750
  • Delivered in as few as 12 weeks
  • Invoiced only on delivery
  • Choose your own cell line background
Single nucleotide polymorphisms (SNP) knock-ins
  • Starting at £9,950/$13,500/€11,650
  • Delivered in as few as 14 weeks
  • Option to define zygosity requirements
iPSC editing
  • Starting at £12,800/$16,950/€14,950
  • Delivered in as few as 12 weeks
  • Fully supported standard knockout and knock-in (SNP, tag, reporter) projects
  • Alkaline phosphatase standard
  • Differentiation services not currently provided
Double knockouts
  • Starting at £15,000/$19,900/€17,500
  • Delivered in as few as 14 weeks
  • Option to include both single knockout clones
Tag knock-ins
  • Starting at £11,650/$15,500/€13,500
  • Delivered in as few as 20 weeks
  • His, FLAG, or Myc options
  • Defined, N-terminal, or C-terminal insert location
Complex edits
  • Internal knowledge and expertise to perform just about any edit in any cell line
  • For complex edits not defined above, please contact us today to enquire about pricing and turnaround time
Cas9 expressing stable lines
  • Starting at £3,750/$4,950/€4,250
  • Delivered in as few as 6 weeks
  • Pooled or clonal format
Reporter knock-ins
  • Starting at £12,800/$16,950/€14,950
  • Delivered in as few as 20 weeks
  • RFP, GFP or customer-defined fluorophore options
  • Also have options for HiBit, LgBit, or NanoLUC insertions
  • Defined, N-terminal, or C-terminal insert location

Project workflow

Cell line engineering project workflow
  • Submit your request via the online Cell Line Configurator Tool or your local Business Development partner.
  • Our expert technical team will review your projects requirements.
  • Your project manager will promptly provide a statement of work outlining timelines and approval process.
  • Once approved, the project will be initiated by our highly qualified cell line engineering team.
  • Throughout the manufacturing process you will receive monthly updates from our Project Management Office.*
  • Once the project is complete, we provide a full project report and confirm shipping details.

*All customers receive project initiation and status updates outlining the on-time progress of their projects. Customers will also have the ability to email queries. If there are any delays, this will be handled by a project manager. A dedicated project manager will be provided for projects with 10+ cell lines.



Manufacturing Workflow

Cell line engineering manufacturing workflow
  • Customers can choose to provide an in-house cell line at no additional charge. During the onboarding process, we perform mycoplasma and sterility testing, as well as assess tranfectability and single cell dilution growth characteristics.
  • At this time, we will also designs and evaluates reagents to be used in the manufacturing process.
  • During the cell line engineering phase the CLE team performs the desired edit defined in the SOW. Throughout the process, our Project Management team provides regular updates and will contact you immediately if any issue or complication should arise during the manufacturing process.
  • After manufacturing is complete, the cell line is validated for the desired edit. As standard, we validate at the genomic level with Sanger sequencing and for our reporter cell lines we provide a functional readout that verifies reporter expression.