Project workflow

- Submit your request via the online Cell Line Configurator Tool or your local Business Development partner.
- Our expert technical team will review your project requirements.
- Your project manager will promptly provide a statement of work outlining timelines and approval process.
- Once approved, the project will be initiated by our highly-qualified cell line engineering team.
- Throughout the manufacturing process you will receive monthly updates from our Project Management Office.*
- Once the project is complete, we provide a full project report and confirm shipping details.
*All customers receive project initiation and status updates outlining the on-time progress of their project. Customers will also have the ability to email queries. If there are any delays, this will be handled by a project manager. A dedicated project manager will be provided for projects with 10+ cell lines.
Manufacturing Workflow

- Customers can choose to provide an in-house cell line at no additional charge. During the onboarding process, we perform mycoplasma and sterility testing, as well as assess transfectability and single cell dilution growth characteristics.
- In parallel with the cell line assessment phase, we also design and evaluate reagents to be used in the manufacturing process.
- During the cell line engineering phase, our team performs the desired edit(s) defined in the statement of work. Throughout the process, our Project Management team provides regular updates and will contact you immediately if any issue or complication should arise during the manufacturing process.
- After manufacturing is complete, the cell line is validated for the desired edit. As standard, we validate at the genomic level with Sanger sequencing and for our reporter cell lines we provide a functional readout that verifies reporter expression.
Licensing and cell lines
Horizon has agreements in place with both ATCC and ECACC, so our clients can work with just about any cell line background. Additionally, we may be able to support securing cell lines from other providers including MSK and NCI.
iPSC editing
Certain drug screening, target validation and toxicity testing methods that utilize in-vitro differentiated disease models may be covered by one or more patents controlled by Evotec International GmbH. Those patents include EP2270196B1, EP1745144B1 and EP3070174B1 and corresponding foreign patents and pending applications. The purchase or transfer of this product is not intended, either expressly or by implication, to grant any right or license to practice under the foregoing patents. Purchasers are advised to contact Evotec International GmbH at info@evotec.com for information regarding a license to practice under such patents.
*Note on inducible cell line projectsHorizon uses the Tet-On 3G inducible expression system for inducible custom engineering projects. This platform is based on the Tet-On® 3G bipartite induction system: a tightly regulated system consisting of an optimized inducible RNA polymerase II promoter (TRE3G), which provides both minimal basal expression and potent activation upon induction with doxycycline. Prior to starting an inducible cell line engineering project, it is the responsibility of the client to provide licensing from Clontech Laboratories for freedom to use this system.
Terms & conditions
All cell line engineering projects are subject to our Terms & Conditions and the Limited Use Label License agreements.