Base editing edits the genome without the risks associated with double strand DNA breaks inherent with first generation CRISPR-Cas9, which can lead to cell toxicity and unwanted genomic rearrangements. However, adopting new technologies can be daunting and take time away from other priorities.
Now you can partner with our in-house Preclinical Services team, who will guide you through the process and perform the experimental work for you, delivering the next-generation cell models you need for your next ground-breaking project.
Revvity’s Pin-point™ Cell Engineering services combine over a decade of expertise in cell line engineering in hundreds of cell types with our unique base editing technology to introduce precision edits into your cell line of choice.
Cell line engineering services with the novel Pin-point base editing platform
Accelerate your research by combining Revvity’s unique Pin-point technology and preclinical services for advanced cell engineering.
- Leverage the power of our unique base editing approach to introduce precise gene edits while avoiding double strand DNA breaks.
- Explore redundant gene functions and multiple gene interactions in your cell model of choice.
- Fast-track your multiplex gene knock-out projects with low-risk simultaneous editing of multiple gene targets.
- Minimize cell toxicity, off-target editing, and unwanted genomic rearrangements.
- Leverage the unique ability of the Pin-point platform for single-intervention, multiplex knockout, and simultaneous knockout and knock-in for generation of highly complex cell models.
- Receive high-quality, reproducible data that you can trust delivered by our expert in-house team.
Partner with Revvity for unrivalled precision and flexibility in your cell engineering projects.
Additional Services
Discuss with our scientific team to learn more about functional validation and verification services available, as well as additional deliverables such as control clones or pools, to be added onto the project.
Example Pin-point Multiple Gene Knock-out Project Workflow
- Reagent delivery conditions are optimised using a highly active control single guide RNA (sgRNA).
- Candidate Pin-point sgRNAs targeting the genes of interest are designed and tested in an arrayed format to identify the gRNAs giving the most efficient gene knock-out.
- Shortlisted sgRNAs are validated individually in larger scale transfections to confirm efficiency.
- Validated sgRNAs are combined in multiplex transfections to generate gene edited pools containing cells with multiple combinations of knock-out edits.
- Clonal cell populations are isolated from the edited pool and DNA sequence analysis is performed to identify single and multiple edited clones.
- Clones with desirable genotypes are expanded and editing is confirmed prior to cryopreservation.
Identifying active Pin-point sgRNAs for gene knockout via C to T base conversion and protein loss screening.
Transfecting 10 sgRNAs/target in HCT-116 cells with Pin-point nCas9 and Rat APOBEC mRNAs under 3 optimized conditions reveals GRN973 and GRN998 as active for genes A and B knockout, confirmed by DNA sequencing (A) and FACS analysis (B). Multiplex transfection of GRN973 and GRN998 with a validated gRNA, GRN762, targeting a third gene, shows no loss of viability in single, double, or triple targeted pools (C). DNA sequence analysis confirms effective C>T conversion across all three genes (D). FACS analysis indicates 7% of transfected cells lost expression of all three target genes (E).
- Optimization of Pin-point reagent delivery conditions to maximize editing efficiency in your cell line(s) of choice.
- Design and screening of candidate Pin-point guide RNAs to enable highly effective C to T editing of your target(s) of interest.
- Potential for simultaneous editing of multiple gene targets through co-delivery of high activity guide RNAs.
- Isolation and genotyping of clonal cell populations, enabling identification of single and multiple edited clones from a single screen.
- Cryopreserved clonal cell lines and supporting data.
- Regular updates from the Project Management team on project progression.
Featured Resources
Complex genome engineering with the Pin-point base editing system, even in sensitive cell types
An application note demonstrating complex genome engineering with the Pin-point base editing system.
CRISPR-derived base editing technology: The what, the how, and the why
Read this DDW article to find out how base editing has emerged as an attractive gene editing option for researchers wanting to develop stem cell-based therapies.