Functional genomic screens enable modulation of hundreds or thousands of genes in a single experiment to identify genetic pathways, cellular processes, novel therapeutic targets, and to genetically profile existing or potential therapeutics. This information can be used to support cell line development, elucidate mechanisms of action, facilitate patient stratification or explore new treatment paradigms.

With integrated solutions for CRISPR knockout, CRISPR activation, CRISPR inhibition, and RNAi technologies our screening portfolio provides you a complete functional genomic screening platform. Using either cell lines or primary cell cultures, Horizon’s screening platform can perform pooled, arrayed and even single cell screens with our flexible library technologies and formats. Libraries can be used either alone, or in tandem, and coupled with a variety of assay readouts.

Arrayed screening

Using synthetic CRISPR and siRNA reagents on a one-gene per well basis with complex assay readouts.

Pooled screening

Employing lentiviral CRISPRko/i/a approaches in highly multiplexed pooled screens.

CRISPR knockout screening services

Probe gene function. Find drug targets. Stratify patients. Ensuring library specificity with algorithm optimized guide RNA.
Custom services available for unique research projects.

siRNA screening services

Benefit from rapid and efficient workflows.

Building on Dharmacon's 20+ years as the world leader in siRNA development, design and synthesis.

RNA-based immune cell screens

Screening services for primary human immune cells. Address clinically relevant biological questions in vitro.

Screening libraries

Flexibility to scale to your needs

Select from whole genome or gene family catalog CRISPR and RNAi libraries or create a custom library in pooled or arrayed format.

Gene modulation technologies in the development of cell based therapies

Learn about the principles, strategies and experimental outcomes of CRISPR screening     

App note: CRISPR-Cas9 screening to advance drug discovery

Read more about how CRISPR-Cas9 is a powerful approach to advance your drug discovery research. Exploring the various options and considerations to help you select the correct screening approach to suit your needs.

App note: Dual CRISPRi/a screens

Read about CRISPR screens for:

  • Drug mechanisms of action
  • Target identification and validation

CRISPRa screening

CRISPR activation offers transient gain-of-function screening using endogenous expression mechanisms.

CRISPRi screening

CRISPR interference screening offers transient loss-of-function screening with the accuracy of the CRISPR guide system.

Dual CRISPRi/a screening

Exploit the power of paired loss-of-function and gain-of-function screening.

Use of parallel CRISPRi and CRISPRa gene modulation to identify drug interactions with paired gene perturbation analysis.

App note: Whole-genome CRISPRa screening

In this application note, we present a human whole-genome CRISPRa platform and demonstrate its effectiveness in identifying resistance genes complementary to those identified through loss-of-function screening. The ability to enhance transcription by several orders of magnitude offers researchers the opportunity to explore resistance mechanisms based on a CRISPRa-driven gain-of-function platform. 

Single-cell CRISPR screening

Identify the genetic interactions that underlie a treatment response with pooled CRISPR screening followed by single cell analysis.

Functional genomic screening with CRISPR technologies

Learn about CRISPR screening and techniques

Linking Genes to Phenotypes with CRISPR Screening

Read a literature review on the multiple CRIPSR screening techniques available for use in your workflow and their benefits

CRISPR screens in primary human T cells

Gain a better understanding of how immune cells function using functional genomic screens.

Podcast: CRISPR screens

Listen to the recent podcast hosted by GEN:

CRISPR Screens Speed Drug Discovery Efforts in Disease Intervention

CRISPR screening from target selection to patient stratification