Quickly determine therapeutic candidates that modulate regulatory T cells in high throughput, leveraging Horizon's comprehensive knowledge in screening. We will obtain in-vitro data from multiple donors of your small molecules or biologics to move your project forward.
Service description | Number of suitable compounds | Catalog Number |
---|---|---|
Standard iTreg Polarization Assay - Mini | 1 to 5 | IMM04-01 |
Standard iTreg Polarization Assay - Midi | 6 to 11 | IMM04-02 |
Standard iTreg Polarization Assay - Maxi | 12 to 17 | IMM04-03 |
Standard iTreg polarization assay readouts and cloud-based data report
- T cell viability
- T cell phenotyping for:CD25, CD127 and Foxp3
- 9 points compound dose response (8 dilutions + vehicle)
- Data points set of quadruplicates
- 3-fold dilution range
- All plotted data and raw data files are provided
- Table of EC50s
- Quality control data for cell isolation cells and internal assay controls
Representative data of the iTreg polarization assay
A) Optimization of naïve CD4+ T cell polarizing condition in the 384-well plate format. Data represent quadruplicate technical repeats cultured from three donors. B) Drug-modulated iTreg polarization by GSK1059615 during polarization reduced the expression of Foxp3. Flow cytometry data represent naïve T cells cultured from 3 donors in quadruplicate replicates. C) Viability assessment of cells treated with GSK1059615 in the iTreg polarization assay.
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