Functional genomic screening with the Pin-point base editing platform enables controlled evaluation of amino acid changes and/or the introduction of premature stop codons in a precise manner. Screens can be used to tile guide RNAs across an entire gene sequence to generate precise C-to-T mutations that can then be used to select the best guide RNA for potential therapeutic applications, identify critical amino acids in structure-function analysis or to test ligand binding relationships in drug candidate screening.
Through a combined lentiviral and mRNA delivery approach, the Pin-point system combines the targeting precision of CRISPR with the controlled editing of specific C-to-T mutations. This enables evaluation of amino acid changes in a controlled manner, without random mutations confounding results. The utilization of a nickase ensures that no DNA double-strand breaks occur and the DNA damage response pathway is not triggered, supporting work in sensitive cell lines and keeping viability high for easier hit selection.
Leverage the precision of Pin-point base editing to make systematic changes in gene sequences to evaluate critical aspects of functionality, drug-gene interactions, and design optimal sequences for amino acid modification and gene knockout.
Pin-point base editing can be used to
- Edit a gene sequence to determine critical amino acids for protein function
- Edit cells that are typically sensitive to DNA damage in wild type CRISPR studies
- Create single or multiplex mutations in cell models without the risk of genomic rearrangements
The Pin-point screening platform uses
- A pooled lentiviral screening approach
- Tiled guide RNAs for C-to-T base changes across an entire target sequence
- Custom guide RNA libraries designed by our team of experts to your gene(s) of interest
- Next-generation sequencing reporting with bioinformatic analysis and hit nominations